Selection of Chromatography Stationary Phases
Peptides and proteins are one of the four fundamental classes of macro-molecules found in nature. They are frequently studied for their potential as therapeutic drugs. Reversed-phase chromatography has become an essential tool for protein purification on a larger scale for investigative studies. To ensure an efficient purification, it is important to understand the difference between flash chromatography and preparative high-pressure liquid chromatography (HPLC) and to carefully select the type of stationary phase used in the technique.