More commonly known as fat or fatty acids, lipids, together with DNA, proteins and carbohydrates, are one of the four fundamental classes of macromolecules found in nature (Walt D et al., 2012). One of the most important functions of lipids is acting as the building blocks of cellular membranes (Subramaniam S et al., 2011). Other major functions of lipids include energy storage, insulation, cellular communication and protection (Fahy E et al., 2009). Lipids have applications in the cosmetic, medicine, food industries and in nanotechnology (Bernie Y, 2007). Bligh E. and Dwyer W. first performed the extraction and purification of lipids from biological materials like fish in 1959 (Bligh E and Dwyer W, 1959). Since then many new techniques have been developed and employed in lipid extraction and purification.
The separation and purification of lipids using chromatography presents unique challenges to the scientists as they are non-polar in nature and are non-chromophores. However, with the advent of Evaporative Light Scattering Detection (ELSD), isolation and purification of lipids has become relatively easy. Several scientists have published their research (along with the method data) on purification of lipids using BUCHI Flash/Prep chromatography instruments. Below are some examples of highly interesting publications using BUCHI chromatography equipment for the separation and purification of lipids.
Lipase-Catalyzed Synthesis of Sugar Esters in Honey and Agave Syrup
Siebenhaller Sascha, Gentes Julian, Infantes Alba, Muhle-Goll Claudia, Kirschhöfer Frank, Brenner-Weiß Gerald, Ochsenreither Katrin, Syldatk Christoph
https://www.frontiersin.org/articles/
Structural Identification of Antibacterial Lipids from Amazonian Palm Tree Endophytes through the Molecular Network Approach
Effect of deuteration on the phase behaviour and structure of lamellar phases of phosphatidylcholines – Deuterated lipids as proxies for the physical properties of native bilayers
Do you have a specific question regarding the purification of lipids? We have specialists who will quickly and efficiently provide you with competent application support or a feasibility study specifically tailored to your needs. Get in contact with our team via application@buchi.com or participate in our advanced chromatography trainings and bring your sample, that we can find the right solution for your specific separation problem. Register for training
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